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KMID : 0880220130510040522
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Journal of Microbiology
2013 Volume.51 No. 4 p.522 ~ p.526
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Comparative assessment of the intracellular survival of the Burkholderia pseudomallei bopC mutant
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Varintip Srinon
Sunsiree Muangman
Nithima Imyaem
Veerachat Muangsombut
Sunee Korbsrisate
Natalie R. Lazar Adler
Edouard E. Galyov
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Abstract
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Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.
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KEYWORD
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Burkholderia pseudomallei, BopC, type III secretion effector, intracellular survival, phagocyte
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